![]() Among anti-cancer compounds, an important class is represented by anti-mitotic drugs, which arrest cell division (mitosis) and induce cell death 8. The drug discovery and cancer research fields have largely benefited from these innovative approaches 6, 7. The analysis of image sequences enables the quantification of relevant parameters, such as the number of cells and their geometric features (e.g., perimeter, area, nuclear position and shape roundness), as well as the assessment of their movement and fate, gaining unique spatio-temporal information on dynamic biological processes 5. ![]() In cell biology studies, time-lapse microscopy advanced methodologies are used to observe the dynamic behavior of single cells over time 4. Many algorithms have been developed to tackle events arising in different video domains, such as abnormality detection for surveillance 1, human action detection 2, and cell population monitoring 3 in several biological and biomedical studies. The dataset is useful for testing and comparing methods for identifying interphase and mitotic events and reconstructing their lineage, and for discriminating different cellular phenotypes.Īdvances in computer in vision, imaging technologies, and computational tools have led to significant results in video event detection and recognition. It contains various annotations (pixel-wise segmentation masks, object-wise bounding boxes, tracking information). It consists of 29 time-lapse image sequences from HeLa, U2OS, and hTERT RPE-1 cells under different experimental conditions, acquired by differential interference contrast microscopy, for a total of 237.9 hours. In this study, we present ALFI, a dataset of images and annotations for label-free microscopy, made publicly available to the scientific community, that notably extends the current panorama of expertly labeled data for detection and tracking of cultured living nontransformed and cancer human cells. Differently from fluorescence microscopy, label-free techniques can be easily applied to almost all cell lines, reducing sample preparation complexity and phototoxicity. For living cells, the task becomes even more arduous as cells change their morphology over time, can partially overlap, and mitosis leads to new cells. Take a look at the DIYs we’ve gathered for you!ĭon’t hide your souvenirs in a scrapbook, make a whole drawer shadow box to enjoy your travel memories! You’ll need an old drawer, a map, your photos and some little memorable pieces: tickets, badges, magnets and cards and stuff like that.Detecting and tracking multiple moving objects in a video is a challenging task. Whole displays with several travel shadow boxes, your personal photos and drawings will make your home more personalized and will create a nice mood. ![]() You may go for beach or city shadow boxes, for nature or big city views and create a mood that you want. It’s an amazing idea to create a wall art that will fit any space and will keep your pleasant memories next to you all the time. What’s now? Looking through your Instagram pics to memorize the cool days? There’s one more way to enjoy the memories, maybe a bit old-fashioned one but it doesn’t require any gadgets: make a travel shadow box! A shadow box is a great piece to display your best pics, some souvenirs that you’ve already bought but don’t need and even small items like tickets, cards and stuff like that. Many of us are going on a holiday soon and some of us have already come back home.
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